Catalytic roles of lysines (K9, K27, K31) in the N-terminal domain in human adenylate kinase by random site-directed mutagenesis
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چکیده
منابع مشابه
N-terminal protease of pestiviruses: identification of putative catalytic residues by site-directed mutagenesis.
Pestiviruses are the only members of the Flaviviridae that encode a nonstructural protease at the N terminus of their polyproteins. This N-terminal protease (Npro) cleaves itself off of the nascent polyprotein autocatalytically and thereby generates the N terminus of the adjacent viral capsid protein C. In previous reports, sequence similarities between Npro and the catalytic residues of papain...
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Perspectives in Biochemistry Mechanism of Adenylate Kinase: Site-Directed Mutagenesis versus X-ray and NMR+
Controversy is an integral part of scientific research and is often a precursor to the truth. However, this lesson has been learned in a very hard way in the case of the structurefunction relationship of adenylate kinase (AK),’ which catalyzes the interconversion between MgATP + AMP and MgADP + ADP. While this small kinase has been considered a “model kinase” and the enzyme-substrate interactio...
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ژورنال
عنوان ژورنال: IUBMB Life
سال: 1996
ISSN: 1521-6543
DOI: 10.1080/15216549600201513